Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues
نویسندگان
چکیده
منابع مشابه
Small interfering RNA; principles, applications and challenges--
Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...
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Multiple gene silencing is being required to target and tangle metabolic pathways in eukaryotes and researchers have to develop a subtle method for construction of RNA interference (RNAi) cassettes. Although, several vectors have been developed due to different screening and cloning strategies but still some potential limitations remain to be dissolved. Here, we worked out a simple cloning stra...
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1. Esengil, H. et al. Nat. Chem. Biol. 3, 154–155 (2007). 2. Jung, D.W. et al. Mol. Biosyst. 1, 85–92 (2005). 3. Khersonsky, S.M. et al. J. Am. Chem. Soc. 125, 11804–11805 (2003). 4. Peterson, R.T. et al. Proc. Natl. Acad. Sci. USA 97, 12965–12969 (2000). 5. Iversen, P.L. & Newbry, S. Curr. Opin. Mol. Ther. 7, 104–108 (2005). 6. Weber, W. & Fussenegger, M. Handb. Exp. Pharmacol. 178, 73–105 (20...
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RNA interference (RNAi) is widespread in eukaryotes and regulates gene expression transcriptionally or post-transcriptionally. In fission yeast, RNAi is tightly coupled to template transcription and chromatin modifications that establish heterochromatin in cis. Exogenous double-stranded RNA (dsRNA) triggers seem to induce heterochromatin formation in trans only when certain silencing proteins a...
متن کاملRNA interference and small interfering RNAs.
The term aRNA interferenceo (RNAi) was coined after the groundbreaking discovery that injection of double-stranded RNA (dsRNA) into the nematode Caenorhabditis elegans leads to specific silencing of genes highly homologous in sequence to the delivered dsRNA. The RNAi phenotype is either identical to the genetic null mutant or resembles an allelic series of mutants. The dsRNA can also be deliver...
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ژورنال
عنوان ژورنال: Molecular Diversity
سال: 2010
ISSN: 1381-1991,1573-501X
DOI: 10.1007/s11030-010-9290-1